Gary W Cline PhD

Associate Professor of Medicine (Endocrinology); Director, Analytical Core, Mouse Metabolic Phenotyping Center at Yale Univ. School of Medicine

Research Interests

diabetes mellitus

Research Summary

Diabetes mellitus has a profound impact on hepatic and peripheral glucose metabolic fluxes. My studies on the biochemical mechanisms responsible for the pathophysiology of diabetes mellitus primarily employ nuclear magnetic resonance spectroscopy, mass spectroscopy, and stable isotopic techniques. Using these techniques, the flux and fate of key intermediates of carbohydrate and lipid metabolism can be determined in vitro and in vivo. These techniques are developed in cell systems and small animal models and when appropriate applied to studies of human physiology. New areas of research are the application and adaptation of these techniques to the study of pancreatic beta-cell metabolism and glucose-stimulated insulin release.

Future Research
Recent dramatic improvement in the success rate of islet transplantation in humans has prompted interest for more widespread application of this methodology. My future research will explore the development of NMR based methods for non-invasive in vitro quality assessment of tissue destined for transplantation, and in vivo monitoring of islet transplants.