Morphology Core Facility
The Morphology Core Facility provides instrumentation and technical expertise for the preparation, acquisition and analysis of images of cells and tissues at the light microscopic level. Given the cost of such instrumentation and the high level of technical expertise required to perform these investigational techniques, this Core was established to ensure the availability of these techniques for Center members. In recognition of the broad usefulness of this Core facility, the School of Medicine has partnered with the Liver Center by making ongoing, major investments to ensure that the facility remains state-of-the-art.
The Core has two confocal microscopes, plus a combined confocal and two-photon microscope. Both of our conventional confocal microscopes are Zeiss LSM 510 laser scanning microscopes. Both have three fluorescence detection channels, and one also has a meta-detector. The newest system is a Zeiss LSM 710 DUO NLO. This component allows two-photon excitation using a Spectra Physics MaiTai titanium sapphire laser. When coupled with the four high sensitivity external detectors (NDDs) the MaiTai is capable of deep tissue imaging. The 710 is also configured with a second “Live” scan head making it able to collect at very rapid acquisition rates. In addition, the stage is fitted with a temperature and CO2 controllable chamber for long term imaging experiments. There are two workstation computers in the facility, one dedicated to confocal image processing and the other for 3D rendering and volumetric analysis with Improvision’s Volocity.
The Core maintains an epifluorescence microscope with a cooled high sensitivity Hamamatsu CCD camera, a camera equipped fluorescence dissecting microscope, a brightfield multi-head microscope with a color CCD camera, and an inverted epifluorescence microscope for ratio imaging. The Core also maintains a microscope for video microscopy. A Leica cryostat is available for frozen tissue sectioning. Interested investigators should contact Dr. Michael Nathanson or Mr. Al Mennone (5-3154) for access to the confocal microscopes, or other light microscopic equipment.
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