BD LSRII at Amistad

UV Laser - 355nm 60 mW

PMT	Bandpass	Long Pass	Fluorochrome
A	740/35	690LP	BUV737
B	450/50	410LP	DAPI, Alexa Fluor 350
C	379/28		BUV395

**DAPI and BUV395 Should not be used together. Additional filters are necessary for Calcium Flux and Side Population detection, please see the staff about filters swaps.

Violet Laser - 405nm 50 mW

PMT	Bandpass	Long Pass	Fluorochrome
A	710/50	690LP	BV711
B	610/20	595LP	BV605, Alexa Fluor 605, QDot605
C	525/50	505LP	Alexa Fluor 430, CFP, Am Cyan, Pacific Orange
D	450/50		Cascade Blue, Pacific Blue, Sky Blue, Alexa Fluor 405, BFP, CFP, Hoechst

Blue Laser - 488nm 50 mW

PMT	Bandpass	Long Pass	Fluorochrome
A	685/35	630LP	PerCP, EMA, PercP-Cy5.5
B	525/50	505LP	FITC, GFP, PKH67, CFSE, Alexa Fluor 488, YFP*
C	488/10		SSC

*YFP-if detecting YFP and GFP ask for filters change on A and B PMT's

Green Laser - 532nm 150 mW

PMT	Bandpass	Long Pass	Fluorochrome
A	780/60	735LP	PE-Cy7, PE-alexa750
B	710/50	685Lp	PE-Cy5.5
C	675/20	630LP	PE-Cy5, PE-Alexa647
D	610/20	600LP	Alexa Fluor 594*, PE-Texas Red, Alexa Fluor 568*, PI, Rhodamine-B, PI
E	575/26		PE, DsRed, RFP, Pyronin-Y, PKH26

*Alexa Fluor 594 only 25% Ex., *Alexa Fluor 568 only 34% Ex.

Red Laser - 640nm 70 mW

PMT	Bandpass	Long Pass	Fluorochrome
A	780/60	755LP	APC-Cy7, Alexa Fluor 750*
B	710/50	690LP	Alexa Fluor 680, Alexa Fluor 700
C	660/20		APC, Alexa Fluor 647

*Alexa Fluor 750 only 18% Ex.