Transgenic and Knock Out Mice
The Flavell laboratory operates a facility that routinely generates transgenic mice by microinjection of blastocysts, and knockout mice by homologous recombination in embryonic stem cells followed by microinjection of the cells. Two full time staff operate this state-of-the-art facility. Many members of the Department of Immunobiology make use of this facility on a collaborative basis with Dr. Flavell.
Flow Cytometry and Cell Sorter Core Facility
Cell sorting instruments are located in several rooms in The Anlyan Center (TAC), the Amistad facility, the 300 George Street facility, and in the LEPH facility. Users must sign-up in each sorter’s calendar, no Internet scheduling is permitted for cell sorting. The core houses many high-speed sorters: the FACSVantage SE, Beckman Coulter MoFlo and Reflection iCyt located in S617 and the FACSAria in LEPH 901B are operated by staff members. The FACS Aria in LEPH 901B is NOW OPEN to sort BSL-1, BSL-2, and BSL-3 materials. All sorters except MoFlo are also equipped for sorting live human, primate or other potentially biohazardous cells. Users of the Sony- iCyt Reflection can take advantage of using two sort heads and a lower charge for the second head to be running. Sorters are located inTAC S613, S533, S633; 300 George Street, 2320H; and Amistad, 416 and 105.
Flow Cytometry Analyzers are strategically placed in nearby laboratories and are also located in several rooms in The Anlyan Center (TAC), the Amistad facility, the 300 George Street facility, and in the LEPH facility. Internet sign up for analysis time is available from any computer via the internet for users after completing the training. Users must log into www.yaleflowscheduling.com using their current schedulebook.com e-mail and password to reserve time on sorters. There are 9 user-operated analyzers in the TAC Building (LSR II, LSRII Green, LSRII TAC5, three FACSCaliburs, three Stratedigm analyzers), LSRII and FACSCalibur in Amistad, and at 300 George Street Room 2320H we have FACSCalibur, Stratedigm-8 and special order LSRII.
Amnis Imagestream-X imaging flow cytometer (TAC, Room S633)- provides users with the ability to gain detailed images of a large number of cells in a relatively short period of time and with the opportunity to perform a range of novel applications including co-localization, internalization, stem cell differentiation, and cell-cell interactions. Machines are checked daily for optimal performance.