Transgenic and Knock Out Mice
The Flavell laboratory operates a facility that routinely generates transgenic mice by microinjection of blastocysts, and knockout mice by homologous recombination in embryonic stem cells followed by microinjection of the cells. Two full time staff operate this state-of-the-art facility. Many members of the Department of Immunobiology make use of this facility on a collaborative basis with Dr. Flavell.
Fluorescence in situ and in vivo MicroscopyThe Department has available multiple fluorescence and confocal microscopes suitable for the detection of up to 6 channels ideal for in situ microscopy of cells and tissues that are shared by individual laboratories. The department has access to an Olympus BX61WI fluorescence microscope equipped with a 20×, 0.95NA water immersion Olympus objective, single-beam LaVision TriM laser-scanning microscope (LaVision Biotec) run with Inspector software, and illuminated with a Chameleon Vision II Ti:Sapphire laser (Coherent) suitable for intravital 2-photon microscopy. The intravital imaging facility is run by David Gonzalez and directed by Dr. Ann Haberman, in the Department of Laboratory Medicine.
The Anlyan Center (TAC)
The Anlyan Center (TAC), constructed at a cost of $176 million, is part of Yale's $1 billion investment for new and reconstructed biomedical research facilities over the next 10 years. The new building-located at the corner of Congress Avenue and Cedar Street-provides six floors of laboratories for disease-oriented research as well as core research resources and teaching facilities, including an animal resources center and a magnetic resonance center.
Sixteen of the 21 faculty in the Department of Immunobiology are housed in TAC and the remaining laboratories are located in nearby buildings, including the Amistad facility, and the George Street facility. This provides Yale Immunology with a unique concentration of superb laboratories and investigators dedicated to the study of the immune system. We believe this represents an outstanding training environment. Four of the faculty are funded by the Howard Hughes Medical Institute (HHMI), which provides for substantial research resources. Overall, the Department of Immunobiology provides perhaps the largest integrated training program in immunology in the country, led by a faculty with a reputation for leadership and excellence in research.
Immunobiology laboratories are fully equipped with all of the standard, modern equipment needed for research in molecular biology and immunology. The Department of Immunobiology maintains a wide variety of major equipment including fluorescence microscopes, scintillation counters, film developer, phosphoimagers, fluorimager, BIAcore apparatus, multiple flow cytometers/cell sorters, cesium irradiator, and an upright laser scanning microscope with a two-photon laser used for real time imaging of cells within lymphoid tissues. Dr. Richard Flavell, chair of the department, oversees a very active transgenic mouse/ES cell/knockout facility to which members of the department have easy access. Also easily available are core facilities in electron microscopy, histology, pathology, mass spectroscopy and monoclonal antibody production. HHMI operates an efficient, low cost oligonucleotide and peptide synthesis facility, which also performs DNA and protein sequencing. A wide variety of additional facilities are provided by Yale University including modern computer technology, access to the internet and excellent libraries.
Media Preparation Facility
The glasswash facility supported by the Howard Hughes Medical Institute prepares media, washes glassware, sterilizes material and disposes of biomedical waste for the laboratories in the Immunobiology Section. Equipment includes a laboratory grade glassware washer, a "clean steam" sterilizer, a small sterilizer and a high temperature oven. The facility has a full-time staff of two and is supervised by an additional HHMI employee. The facility prepares standard and custom tissue culture media, bacterial media and plates and sterile salt solutions.