Patrick G Gallagher MD
Professor of Pediatrics (Neonatology), of Genetics and of Pathology; Director, Yale Center for Blood Disorders; Director, Expression and Genomics Core, Yale Center of Excellence in Molecular Hematology
Research Interests
Neonatal hematology; Erythropoiesis; Inherited abnormalities of the erythrocyte including metabolic, membrane, and hemoglobin disorders; Sickle cell disease; Hereditary spherocytosis; Elliptocytosis; Pyropoikilocytosis; Stomatocytosis
Research Summary
Dr. Gallagher’s research focuses on the genetic basis of inherited disorders of the erythrocyte and the molecular control of erythropoiesis. Dr. Gallagher’s research is funded by the NIH and private foundations. Current NIH support includes two R01 awards, ARRA supplements, and P30 support for the Yale Center for Excellence in Molecular Hematology (YCEMH). Dr. Gallagher is PI of the Expression and Genomics Core of the YCEMH. He is also supported by an Innovation in Clinical Research Award from the Doris Duke Foundation. Dr. Gallagher is fully engaged in the Yale scientific community, with a joint appointment in Genetics, and active membership in both the Yale Stem Cell Center and the Yale Cancer Center. He is director of the Yale Center for Blood Disorders.
Current extramural activities include Editorial Board service for the Journal of Biological Chemistry, the American Journal of Hematology, Pediatric and Developmental Pathology, BMC Pregnancy and Childbirth (Associate Editor), and Blood Cells, Molecules and Disease and grant and abstract reviewing for numerous NIH, private foundations, and academic society meetings including PAS. He recently finished a two-year-term as chair of the NIH Erythrocyte and Leukocyte Biology Study Section and is currently chair of the American Society of Hematology Scientific Subcommittee on Hemoglobin/Red Cell.
Extensive Research Description
Dr. Gallagher has trained numerous undergraduate, graduate and medical students, MD and PhD postdoctoral fellows, and sabbatical faculty. Laboratory graduates hold positions throughout the world and trainees have received awards and honors from local, regional, and national organizations. In addition to this T32, he participates in training grants in Genetics, Pathology and Medicine. He serves on the Department of Pediatrics Scholarship Oversight Committee (SOC). He has served on numerous individual SOCs as member or chair, as well as various medical and graduate school mentoring committees, PhD thesis committees, etc. Directing the Perinatal Medicine fellowship is a vital part of Dr. Gallagher’s activities as a Yale Medical School faculty member.
Selected Publications
- Laflamme K, Owen AN, Devlin EE, Yang MQ, Wong C, Steiner LA, Garrett LJ, Elnitski L, Gallagher PG, Bodine DM.Functional analysis of a novel cis-acting regulatory region within the human ankyrin gene (ANK-1) promoter. Mol Cell Biol. 2010 Jul;30(14):3493-502. Epub 2010 May 17. PMID:20479128
- Li D, Harper SL, Tang HY, Maksimova Y, Gallagher PG, Speicher DW. A comprehensive model of the spectrin divalent tetramer binding region deduced using homology modeling and chemical cross-linking of a mini-spectrin. J Biol Chem. 2010 Sep 17;285(38):29535-45. Epub 2010 Jul 6.PMID: 20610390
- Yang MQ, Laflamme K, Gotea V, Joiner CH, Seidel NE, Wong C, Petrykowska HM, Lichtenberg J, Lee S, Welch L, Gallagher PG, Bodine DM, Elnitski L. Genome-wide detection of a TFIID localization element from an initial human disease mutation. Nucleic Acids Res. 2011 Mar;39(6):2175-87. Epub 2010 Nov 11.PMID: 21071415
- Gallagher PG, Steiner LA, Liem RI, Owen AN, Cline AP, Seidel NE, Garrett LJ, Bodine DM. Mutation of a barrier insulator in the human ankyrin-1 gene is associated with hereditary spherocytosis. J Clin Invest. 2010 Dec 1;120(12):4453-65. Epub 2010 Nov 22.PMID: 21099109
- Isern J, He Z, Fraser ST, Nowotschin S, Ferrer-Vaquer A, Moore R, Hadjantonakis AK, Schulz V, Tuck D, Gallagher PG, Baron MH. Single-lineage transcriptome analysis reveals key regulatory pathways in primitive erythroid progenitors in the mouse embryo. Blood. 2011 May 5;117(18):4924-34. Epub 2011 Jan 24.PMID:21263157
- Hopewell B, Steiner LA, Ehrenkranz RA, Bizzarro MJ, Gallagher PG. Partial exchange transfusion for polycythemia hyperviscosity syndrome. Am J Perinatol. 2011 28(7):557-64. Epub 2011 Mar 1.PMID:21365533
- Massaro SA, Bajaj R, Pashankar FD, Ornstein D, Gallagher PG, Krause DS, Li P. Pediatr Blood Cancer. Bi-allelic deletions within 13q14 and transient trisomy 21 with absence of GATA1s in pediatric acute megakaryoblastic leukemia. 2011; 57(3):516-9.Epub 2011 Apr 29. PMID:21538823
- Li X, Wang S, Li Y, Deng C, Steiner LA, Xiao H, Wu C, Bungert J, Gallagher PG, Felsenfeld G, Qiu Y, Huang S. Chromatin boundaries require functional collaboration between the hSET1 and NURF complexes. Blood. 2011;118(5):1386-94. Epub 2011 Jun 8. PMID: 21653943
- Bouyer G, Cueff A, Egée S, Kmiecik J, Maksimova Y, Glogowska E, Gallagher PG, Thomas SL. Erythrocyte peripheral type benzodiazepine receptor/voltage-dependent anion channels are upregulated by Plasmodium falciparum.Blood. 2011 Aug 25;118(8):2305-12. Epub 2011 Jul 27. PMID:21795748
- Stewart AK, Shmukler BE, Vandorpe DH, Rivera A, Heneghan JF, Li X, Hsu A, Karpatkin M, O'Neill AF, Bauer DE, Heeney MM, John K, Kuypers FA, Gallagher PG, Lux SE, Brugnara C, Westhoff CM, Alper SL. Loss-of-function and gain-of-function phenotypes of stomatocytosis mutant RhAG F65S. Am J Physiol Cell Physiol. 2011 Aug 17. [Epub ahead of print] PMID:21849667
Selected Publications
- Laflamme K, Owen AN, Devlin EE, Yang MQ, Wong C, Steiner LA, Garrett LJ, Elnitski L, Gallagher PG, Bodine DM.Functional analysis of a novel cis-acting regulatory region within the human ankyrin gene (ANK-1) promoter. Mol Cell Biol. 2010 Jul;30(14):3493-502. Epub 2010 May 17. PMID:20479128
- Li D, Harper SL, Tang HY, Maksimova Y, Gallagher PG, Speicher DW. A comprehensive model of the spectrin divalent tetramer binding region deduced using homology modeling and chemical cross-linking of a mini-spectrin. J Biol Chem. 2010 Sep 17;285(38):29535-45. Epub 2010 Jul 6.PMID: 20610390
- Yang MQ, Laflamme K, Gotea V, Joiner CH, Seidel NE, Wong C, Petrykowska HM, Lichtenberg J, Lee S, Welch L, Gallagher PG, Bodine DM, Elnitski L. Genome-wide detection of a TFIID localization element from an initial human disease mutation. Nucleic Acids Res. 2011 Mar;39(6):2175-87. Epub 2010 Nov 11.PMID: 21071415
- Gallagher PG, Steiner LA, Liem RI, Owen AN, Cline AP, Seidel NE, Garrett LJ, Bodine DM. Mutation of a barrier insulator in the human ankyrin-1 gene is associated with hereditary spherocytosis. J Clin Invest. 2010 Dec 1;120(12):4453-65. Epub 2010 Nov 22.PMID: 21099109
- Isern J, He Z, Fraser ST, Nowotschin S, Ferrer-Vaquer A, Moore R, Hadjantonakis AK, Schulz V, Tuck D, Gallagher PG, Baron MH. Single-lineage transcriptome analysis reveals key regulatory pathways in primitive erythroid progenitors in the mouse embryo. Blood. 2011 May 5;117(18):4924-34. Epub 2011 Jan 24.PMID:21263157
- Hopewell B, Steiner LA, Ehrenkranz RA, Bizzarro MJ, Gallagher PG. Partial exchange transfusion for polycythemia hyperviscosity syndrome. Am J Perinatol. 2011 28(7):557-64. Epub 2011 Mar 1.PMID:21365533
- Massaro SA, Bajaj R, Pashankar FD, Ornstein D, Gallagher PG, Krause DS, Li P. Pediatr Blood Cancer. Bi-allelic deletions within 13q14 and transient trisomy 21 with absence of GATA1s in pediatric acute megakaryoblastic leukemia. 2011; 57(3):516-9.Epub 2011 Apr 29. PMID:21538823
- Li X, Wang S, Li Y, Deng C, Steiner LA, Xiao H, Wu C, Bungert J, Gallagher PG, Felsenfeld G, Qiu Y, Huang S. Chromatin boundaries require functional collaboration between the hSET1 and NURF complexes. Blood. 2011;118(5):1386-94. Epub 2011 Jun 8. PMID: 21653943
- Bouyer G, Cueff A, Egée S, Kmiecik J, Maksimova Y, Glogowska E, Gallagher PG, Thomas SL. Erythrocyte peripheral type benzodiazepine receptor/voltage-dependent anion channels are upregulated by Plasmodium falciparum.Blood. 2011 Aug 25;118(8):2305-12. Epub 2011 Jul 27. PMID:21795748
- Stewart AK, Shmukler BE, Vandorpe DH, Rivera A, Heneghan JF, Li X, Hsu A, Karpatkin M, O'Neill AF, Bauer DE, Heeney MM, John K, Kuypers FA, Gallagher PG, Lux SE, Brugnara C, Westhoff CM, Alper SL. Loss-of-function and gain-of-function phenotypes of stomatocytosis mutant RhAG F65S. Am J Physiol Cell Physiol. 2011 Aug 17. [Epub ahead of print] PMID:21849667
- Pilon AM, Ajay SS, Kumar SA, Steiner LA, Cherukuri PF, Wincovitch S, Anderson SM, Comparative Sequencing Program N, Mullikin JC, Gallagher PG, Hardison RC, Margulies EH, Bodine DM. Genome-wide ChIP-Seq reveals a dramatic shift in the binding of the transcription factor erythroid Kruppel-like factor during erythrocyte differentiation. Blood. 2011 Sep 6 PMID:21900194
- Steiner LA, Schulz VP, Maksimova Y, Wong C, Gallagher PG. Patterns of histone H3 lysine 27 monomethylation and erythroid cell-type specific gene expression. J Biol Chem. 2011 Sep 21. [Epub ahead of print] PMID: 21937433
- Houston BL, Zelinski T, Israels SJ, Coghlan G, Chodirker BN, Gallagher PG, Houston DS, Zarychanski R. Refinement of the hereditary xerocytosis locus on chromosome 16q in a large Canadian kindred. Blood Cells Mol Dis. 2011 Sep 22. [Epub ahead of print] PMID:21944700
- Steiner LA, Ehrenkranz RA, Peterec SM, Steiner RD, Reyes-Múgica M, Gallagher PG. Perinatal onset mevalonate kinase deficiency. Pediatr Dev Pathol. 2011 14(4):301-6. Epub 2011 Mar 22. PMID: 21425920
- Yang MQ, Laflamme K, Joiner CH, Seidel NE, Wong C, Petrykowska HM, Gallagher PG, Bodine DM, Elnitsk I L: Genome-wide detection of a TFIID localization element from an initial human disease mutation. Nucl Acid Res 2010
- Gallagher PG, Steiner LA, Liem RI, Owen AN, Cline AP, Seidel NE, Garrett LJ, Bodine DM: Hereditary spherocytosis due to mutation in a barrier insulator in the human ankyrin-1 gene. J Clin Invest 2010; In press
- Rinehart J, Gulcicek EE, Joiner CH, Lifton RP, Gallagher PG: Determinants of erythrocyte hydration. Curr Opin Hematol 2010;17:191-7. PMID: 20182354
- Stewart AK, Vandorpe DH, Heneghan JF, Chebib F, Stolpe K, Akhavein A, Shmukler BE, Gallagher PG*, Alper SL*: co-senior and corresponding authors.The novel, glycophorin A (GPA)-dependent, spherostomatocytosis mutant AE1 E758K induces GPA-independent, endogenous cation transport in amphibian oocytes. Am J Physiol- Cell Physiol 2010; 298:C283-97. PMID: 19907019
- Steiner LA, Maksimova Y, Shultz V, Wong C, Mahajan MC, Weissman SM, Gallagher PG: Chromatin architecture and transcription factor binding regulate expression of erythrocyte membrane protein genes. Mol Cell Bio 2009; 29:5399-412. PMID: 19687298
- Gallagher PG, Nilson DG, Steiner LA, Maksimova YD, Lin JY, Bodine DM (Feb 2009) An insulator with barrier-element activity promotes alpha-spectrin gene expression in erythroid cells., Blood, 113(7)1547-54
- Pilon AM, Arcasoy MO, Dressman HK, Vayda SE, Maksimova YD, Sangerman JI, Gallagher PG, Bodine DM (Dec 2008) Failure of terminal erythroid differentiation in EKLF-deficient mice is associated with cell cycle perturbation and reduced expression of E2F2., Molecular and cellular biology, 28(24)7394-401
- Tolpinrud W, Maksimova YD, Forget BG, Gallagher PG (Nov 2008) Nonsense mutations of the alpha-spectrin gene in hereditary pyropoikilocytosis., Haematologica, 93(11)1752-4
- Mohandas N, Gallagher PG (Nov 2008) Red cell membrane: past, present, and future., Blood, 112(10)3939-48
