Scott Alan Holley, PhD

Associate Professor of Molecular, Cellular, and Developmental Biology

Departments & Organizations

Biochemistry, Biophysics and Structural Biology: Cell Cycle and Signal Transduction

Molecular Cell Biology, Genetics and Development: Developmental Biology | Genetics and Genomics

Education & Training

PhD University of Chicago,
Postdoctoral Fellow Max Planck Institut für Entwicklungsbiologie, Tübingen, Germany

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Contact Info

Scott Alan Holley, PhD
Lab Location
Kline Biology Tower
219 Prospect Street, Ste 1038

New Haven, CT 06511
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Office Location
Kline Biology Tower
219 Prospect Street, Ste 1034

New Haven, CT 06511
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Mailing Address
Dept. of MCDB
P.O. Box 208103

New Haven, CT 06520-8103

Holley Lab

Research Image 1

Figure 1. To the left is a photograph of a live zebrafish embryo during the first day of development. Somites are the repeated structures that give rise to the vertebral column and skeletal muscle. New somites are created in the tailbud, which is also the leading edge of the extending trunk and tail. The schematic to the right summarizes differences in the flow of migrating cells in the blue, green and cyan regions of the tailbud.

Research Image 2

Figure 2. Cell movement within the tailbud was imaged, cells were tracked and average cell velocities over a 10 micron radius were calculated in 3D and projected onto a 2-D surface. The warmer colors indicate regions of higher cell velocities. The arrows indicate 2-D projection of the averaged 3-D velocity vectors.

Research Image 3

Figure 3. High-resolution fluorescent in situ hybridization of the oscillating expression of two segmentation clock genes her1 (green) and deltaC (red). Nuclei are blue. These stripes of gene expression sweep though the tissue in a reiterated, wave-like fashion from posterior (right) to anterior (left). This striped pattern created by the “segmentation clock” presages the segmental pattern of morphological somites and, ultimately, the vertebral column.