Project development, genome editing, gene targeting, transgenics
Senior Research Scientist in Comparative Medicine; Co-director, Yale Genome Editing Center
Yale Genome Editing Center
Services and Resources for Creating Genetically Engineered Mice
YGEC creates genetically engineered mice via CRISPR/Cas genome editing, gene targeting via homologous recombination in ES cells, and traditional transgenic mouse technology. YGEC also cryopreserves sperm or embryos of inherently valuable genetically altered mouse strains. Assisted reproduction technologies include strain reanimation or rescue via in vitro fertilization and/or embryo transfer.
CRISPR/Cas Genome Editing
Creation of genetically engineered mouse strains via CRISPR/Cas. Knockouts, knockins, point mutations, conditional (floxed) alleles.
Pronuclear microinjection of transgene constructs to create transgenic mouse lines.
Creation of genetically altered ES cell lines by homologous recombination. Karyotyping. Generation of chimeric mice by injection of ES cells into blastocysts; transmission of the targeted gene through the germline.
Cryopreservation and storage of genetically altered mouse strains. Reanimation of cryopreserved strains via in vitro fertilization (IVF).
Freezing and storage of mouse embryos.
Call to discuss other embryonic stem cell and mouse embryology-related services.
Contact Us
Embryo and sperm manipulation (e.g. microinjection, zygote electroporation, cryopreservation, rederivation, IVF)
Research Associate 4, MS; Co-Director, Yale Genome Editing Center (YGEC)
Genome editing project development
Senior Research Scientist; Director, Diabetes Endocrinology Research Center Molecular Core
Publication Acknowledgment
If research supported by this Research Core results in publication, please acknowledge this support by including the following in your publication(s): "We thank the Yale Genome Editing Center for their assistance with _________ service.”