TIRF microscopy provides selective illumination of the lower surface of the cells, near the glass/medium interface. Thus, it is possible to visualize processes that occur at the basa plasma membrane, such as endocytosis, exocytosis and cell adhesion, with unmatched signal-to-noise. Moreover, the availability of multiple laser lines enables simulataneous visualization of multiple markers of these processes. This is key to understanding how the variousl molecular components implicated in endocytosis are orchestrated, as well as how coordination between endocytosis and other biological processes at the surface of the cell is achieved.
For questions or to set up a time to use the TIRF Microscope, please contact Stacy Wilson at 737-4311 or firstname.lastname@example.org