Keming Zhou, Ph.D.

Ph.D. in Biophysics from Huazhong University of Science and Technology, China

Flipped SNAREs fuse whole cells, and provide a system where the role of each membrane fusion component can quantitatively be individually determined.

With this cell-based approach, the fusing membranes are available substrates for patch-clamp analysis.

Capacitance and conductance recordings upon membrane fusion in flipped SNAREs system can deconstruct and define the roles of the SNARE fusion machinery and companion proteins in electrophysiology recordings, while providing quantitative kinetic measurements of their effects.