We offer a startup package for electron tomography. The purpose is to provide investigators with an opportunity to try out this new technique and determine whether it would be a good addition to their current portfolio of conventional electron microscopy projects. The package includes generating two tomograms at both high and low magnification levels. We will prepare sample grids, collect image data from the high tilting range, process acquired images, and reconstruct data into 3-dimensional tomograms. The flat-rate charge is $250 for the first image data set (single-axis). Subsequent tomograms will be $200 for a single-axis tilt and $350 for a dual-axis collection.
Biological EM facility provides the following services:
- Sample processing, embedding in plastic resin
- Ultramicrotomy of plastic sections
- Cryo-sectioning and immuno-gold labeling
- Negative staining
- Immuno-electron microscopy
- Sputter coating
- Assisting in transmission electron microscopy and digital imaging
- Consultation and assistance in design of EM experiments
- Electron tomography: tilt series acquisition, image alignment and reconstruction for plastic embedded samples
Cryo EM facility service:
This facility is a "user facility" and currently does not provide cryo EM service. Any parties interested in having samples evaluated for suitability for cryo EM work should contact EM facility director, Xinran Liu
Regular services are invoiced monthly after a project is completed.
|Staff Time / hour||$65||$78||$130|
|Samples embedding (1-4)||$250||$300||$500|
|Samples embedding (4+/each)|| $80
|Plastic sectioning (each)||$72||$86||$144|
|Cryo sample sectioning (run) **||$125||$150||$250|
|Immuno-gold labeling (run) ***
|Grid metal staining (each, 8 minimum)||$6||$8||$12|
|Negative staining (run, 4 max)||$70||$84||$140|
|High pressure freezing /Per shot + LN2
|Freeze substitution / 1-4 samples||$180||$216||$360|
|**The charge for cryo-sectioning is for a maximum of 12 grids, additional grids being charged per time.
***Up to 3 antibodies
Services are invoiced monthly after a project is completed.
|Standard TEM / sample||$350||$420||$700|
|High pressure freezing & freeze-substitution / run||$420||$504||$840|
|Cryosectioning and immunogold labeling / run||$450||$540||$900|
Complete service includes embedding, ultrathin sectioning/cryosectioning, staining / immunogold labeling, observation at the EM, and digital imaging, all carried out by the staff of the facility.
For more information regarding additional services, please contact the facility staff.
Training is invoiced in full once the request has been submitted and approved by the Facility Director, Xinran Liu.
|Electron Microscope||Training Hours||Prepaid Hours||Yale||Other Institution||Industry|
|FEI Tecnai Biotwin||10||6||$1,000||$1,200||$2,000|
|FEI Tecnai T12||10||6||$1,000||$1,200||$2,000|
|FEI Tecnai TF20 TEM/STEM (hourly)
|FEI Tecnai F20 Cryo EM (hourly)
Invoicing & Payment
All requests for services and training must be submitted with a valid Yale PTAEO or purchase order from your institution. Please keep in mind that no work can begin prior to receipt of the PTAEO or purchase order - which will be referenced by the Electron Microscopy to invoice your department or institution.
Please note that in some instances a record of estimated charges (quote) has been emailed to you as requested. This quote is not an invoice. Invoices are emailed/mailed monthly and generally within 30 days after the completion of the requested service. All invoice payments should be made upon receipt of the invoice.
Invoicing and Payment Information
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Mail Payments To:
Make Check Payments To:
Yale University School of Medicine
Invoice or Payment Inquiries: