Instruments

The facility has two point scanning laser confocal microscopes, a Zeiss LSM 510 and a Zeiss LSM 510 Meta. In addition, the facility also has a combined confocal/two-photon Zeiss LSM 710 Duo NLO.

The center also has three image workstations.

Leica SP5

The SP5 has 5 channel detectors, allowing for up to five dyes for simultaneous scanning. The system has been upgraded with a new workstation and replacement of two of the PMT detectors with Leica's new HyD detectors. These detectors have the advantage of very low noise and high sensitivity. The system is equipped with four lasers, 405nm, multi line Argon, 561nM and 633nm.

 

Zeiss LSM 510 Meta

The Zeiss LSM 510 Meta allows discrimination between fluorophores with closely spaced or overlapping emission spectra due to its "meta detector". The meta feature records a complete spectrum at each pixel of an image allowing sophisticated spectral un-mixing to be performed. This microscope is equipped with 458, 477, 488, 514, 543, and 633nm excitation wavelengths and three detector channels, one of which is the meta detector. This allows the imaging of a wide variety of fluorescent probes among them green fluorescent proteins such as GFP, CFP, YFP, and Ds-Red.

 

Zeiss LSM 710 Duo NLO/Multiphoton Microscope

This microscope’s point scanner uses an argon laser and 2 HeNe lasers for excitation (at 458, 488, 514, 543, and 633 nm) and also has three detection channels. The 7-Live scanner is a line scanner capable of rapid acquisition, it is equipped with 405, 488 and 561nm laser lines. This microscope is adapted to examine both fixed and live specimens. The point scanner and line scanner can be used simultaneously for a variety of photo-manipulation experiments such as FRAP, FLIP, FRET, spectral analysis and uncaging.

This system is also equipped for multiphoton fluorescence imaging. Multiphoton imaging is particularly useful for imaging of fluorescence deep into tissues (up to 200 micrometers) with bleaching limited to focal planes, and for flash photolysis studies that require imaging in precise subcellular regions. The setup includes a Spectra-Physics MaiTai titanium:sapphire laser for two photon excitation. The output wavelength of the MaiTai is tunable from ~700-1020 nm. The system has four external non-descanned detectors.

Computer workstations

Three computer workstations (Dell and Macintosh computers) are available in the facility for off-line analysis and 3D reconstruction of images. They are equipped with the Zeiss LSM510 and Zen programs, Volocity program for 3-4 D reconstruction and volumetric analysis and Photoshop software.

Reservation of all resources is done through schedulebook.com. http://www.schedulebook.com/