The facility has three point scanning laser confocal microscopes, a Leica SP5, a combined confocal/two-photon Zeiss LSM 710 Duo NLO and a Leica SP8 Gated STED 3X super resolution microscope. The center also has three image workstations.
The SP5 has 5 channel detectors, allowing for up to five dyes for simultaneous scanning. The system has been upgraded with a new workstation and replacement of two of the PMT detectors with Leica's new HyD detectors. These detectors have the advantage of very low noise and high sensitivity. The system is equipped with four lasers, 405nm, multi line Argon, 561nM and 633nm.
Leica SP8 Gated STED 3X Super Resolution
The Leica TCS SP8 Gated STED 3X super-resolution microscope is based on the STED (STimulated Emission Depletion) principle developed by Nobel Prize laureate Stefan Hell. It provides fast, intuitive, and purely optical access to study subcellular architecture and dynamics at the nanoscale. Resolution can be increased 5 fold compared to diffraction limited confocal systems.
The microscope is equipped with a 405nm laser and a tunable (460nm-660nm) pulsed white light laser for excitation. Two PMT detectors and two HyD detectors are available for tunable spectral detection. Three STED depletion lasers (592nm, 660nm, and 775nm) make 3 dye super-resolution possible. For live cell imaging a temperature and CO2 controlled chamber is available along with a resonate scanner which is capable of scan speeds 10x faster than traditional galvanometric scanners.
The Leica LAS-X software interacts seamlessly with Huygens STED deconvolution software for image enhancement. Users can quickly send their images to Huygens which will increase contrast, suppress noise and increase resolution in X, Y and Z. This software is also available on our workstation computer.
For a detailed description of this system and it's capabilities please visit: http://tinyurl.com/hsek9wt
Zeiss LSM 710 Duo NLO/Multiphoton Microscope
This microscope’s point scanner uses an argon laser and 2 HeNe lasers for excitation (at 458, 488, 514, 543, and 633 nm) and also has three detection channels. The 7-Live scanner is a line scanner capable of rapid acquisition, it is equipped with 405, 488 and 561nm laser lines. This microscope is adapted to examine both fixed and live specimens. The point scanner and line scanner can be used simultaneously for a variety of photo-manipulation experiments such as FRAP, FLIP, FRET, spectral analysis and uncaging.
This system is also equipped for multiphoton fluorescence imaging. Multiphoton imaging is particularly useful for imaging of fluorescence deep into tissues (up to 200 micrometers) with bleaching limited to focal planes, and for flash photolysis studies that require imaging in precise subcellular regions. The setup includes a Spectra-Physics MaiTai titanium:sapphire laser for two photon excitation. The output wavelength of the MaiTai is tunable from ~700-1020 nm. The system has four external non-descanned detectors.
Three computer workstations are available in the facility for off-line analysis and 3D reconstruction of images. They are equipped with the Zeiss LSM510 and Zen programs, Volocity program for 3-4D reconstruction and volumetric analysis and Leica LAS-X / Huygens deconvolution software.
Reservation of all resources is done through schedulebook.com. http://www.schedulebook.com/