The purpose of the Yale Genome Editing Center (YGEC) is to create (and through cryopreservation, maintain) transgenic and knockout models for Yale investigator research. It offers mouse embryo cryopreservation as an economical alternative to maintaining genetically unique colonies of mice. The YGEC has brought together individuals with many years of experience in mouse embryo development, embryo microsurgery, ES cell derivation and maintenance, and embryo cryopreservation. This has allowed users to enjoy a very high success rate for their research projects and obviates the duplication of equipment and training that would occur without such a resource.
Specific services of the YGEC Resource include pronuclear microinjection of mouse zygotes to create transgenic strains; electroporation and selection of mouse embryonic stem (ES) cells; karyotyping of successfully targeted ES clones; microinjection of ES cells into mouse blastocysts; breeding chimeric mice for germ line transmission; and cryoperservation of 2.5d mouse embryos.
Investigators can use AGS to create direct analogs of mutations involved in human disease, thus providing models that can be studied in a way that human subjects cannot. The ability to create such induced mutants allows investigators to effectively use mouse genetics to dissect and analyze complicated developmental pathways, biochemical processes, and biomolecular interactions in a mammal to a level previously unattainable before such technology existed. This enables researchers to investigate biological processes at a far deeper level than in vitro methods such as biochemistry or cell culture will allow, and through the appearance of novel phenotypes resulting from induced mutations, discover new relationships between these processes and pathways.
James McGrath, MD, PhD, Co-Director
Timothy Nottoli, PhD, Co-Director