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Suguru Imaeda MD

Assistant Professor of Dermatology; Director of Dermatology, Yale University Health Services; Director of Dermatology Service, Veteran Affairs Medical Center


Research Summary

Identification of major histocompatibility complex class I (MHC I) associated tumor-specific peptides and immunomodulation of T cell tumors. 2) MHC I antigen processing and presentation in cutaneous T cell lymphoma (CTCL) cells. The research involves isolation, purification and characterization of MHC I self-peptides from a murine and human % cell lymphoma cell lines. Immunoaffinity purification, reversed phase high performance liquid chromatography (RP-HPLC), automated chemical sequencing and peptide synthesis have been used to identify and characterize octamer and nonamer self-peptides from a murine B cell lymphoma cell line. Similar techniques are used to isolate distinctive peptide antigens of CTCL cells and murine T cell tumors. The biological significance of isolated antigens is determined by cytotoxicity assays.

Extensive Research Description

Identification of major histocompatibility complex class I (MHC I) associated tumor-specific peptides and immunomodulation of T cell tumors. 2) MHC I antigen processing and presentation in cutaneous T cell lymphoma (CTCL) cells. The research involves isolation, purification and characterization of MHC I self-peptides from a murine and human % cell lymphoma cell lines. Immunoaffinity purification, reversed phase high performance liquid chromatography (RP-HPLC), automated chemical sequencing and peptide synthesis have been used to identify and characterize octamer and nonamer self-peptides from a murine B cell lymphoma cell line. Similar techniques are used to isolate distinctive peptide antigens of CTCL cells and murine T cell tumors. The biological significance of isolated antigens is determined by cytotoxicity assays.


Selected Publications

  • Girardi M, Duncan KO, Tigelaar RE, Imaeda S, Watsky KL, McNiff JM. Cross-comparison of patch test and lymphocyte proliferation responses in patients with a history of acute generalized exanthematous pustulosis. Am J Dermatopathol. 2005, 27:343-346.
  • Berger, C.L., Longley, B.J., Imaeda, S., Christensen, I., Heald, P., and Edelson, R.L. Tumor-specific peptides in cutaneous T cell lymphoma: association with class I MHC and possible derivation from the clonotypic T cell receptor. Int J Cancer. 1998, 76:304-311.
  • Imaeda, S., Felli, A., Schmitt, I., Chimenti, S., and Edelson, R.L. Induction of functional empty class I major histocompatibility complex glycoproteins by photoactivated 8-methoxypsoralen. J Invest Dermatol. 1996, 107:887-890.
  • Shepherd, J.C., Schumacher, T.N.M., Ashton-Rickardt, P.G., Imaeda, S., Ploegh, H.L., Janeway, C.A., and Tonegawa, S. TAP1-dependent peptide translocation in vitro is ATP-dependent and peptide selective. Cell, 1993, 74:577-584.

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