7b. Two Group T-test Comparisons

Generate two separate .msb files for each group as seen in the Multisubject Manual Page.

1. Choose dualmultisubject from the BioImageSuite main menu. Three windows will appear: a Transform Viewer, a Reference Viewer and a BrainRegister menu bar. On the BrainRegister menu bar choose the first (Multisubject | Base). A new MultiSubject Tool window will appear.
2. On the new MultiSubject Tool window choose (Setup | Load Setup).
3. Choose your generated .msb file for your first group and click Open.
4. On the MultiSubject Tool window choose the Results Tab. Choose your task of interest from the list of tasks then click Change Current Task.
5. On the MultiSubject Tool window click (Images | Load All Images).
6. On the MultiSubject Tool window click Compute Average Functional.
7. On the BrainRegister menu bar choose the second (Multisubject2 | Base). A second MultiSubject Tool window will appear.
8. On the second MultiSubject Tool window choose (Setup | Load Setup).
9. Choose your generated .msb file for your second group and click Open.
10. On the MultiSubject Tool window choose the Results Tab. Choose your task of interest from the list of tasks then click Change Current Task.
11. On the MultiSubject Tool window click (Images | Load All Images).
12. On the MultiSubject Tool window click Compute Average Functional.
13. On either Multisubject window click the Base tab and under the Reference window click Display Reference.
14. On either Multisubject window under Comparisons click Show Compare Tool. A new Registration/Overlay Tool window will appear and it will be on the Compare Image tab. The filename boxes will be empty.
15. From the first Multisubject window (Group 1) under Comparisons click Send Mean Functional to Compare Tool as Set1. This will fill in the first filename box on the Registration/Overlay Tool window.
16. From the second Multisubject window (Group 2) under Comparisons click Send Mean Functional to Compare Tool as Set2. This will fill in the second filename box on the Registration/Overlay Tool window.
17. On the Registration/Overly Tool window click Compute Tmap. The computed functional image will be displayed in the transform window.
18. On the Registration/Overlay Tool window click the Functional Overlay tab.
19. Choose your threshold and enter it into the Low Threshold box. Refer to instructions for 5a (steps 4-8) for an explanation on how to choose a threshold.
20. On the Registration/Overlay Tool window click Create Overlay.