Departments & Organizations
Cellular & Molecular Physiology: Graduate Program in Cellular and Molecular Physiology | Membrane Biophysics | Membrane Proteins - Ion Channels | Membrane Proteins - Pumps and Transporters | Physiology and Integrative Medical Biology Track
Yale Combined Program in the Biological and Biomedical Sciences (BBS): Microbiology: Molecular Genetics | Molecular Medicine, Pharmacology, and Physiology: Ion Channels, Pumps and Transporters; Membrane Biology and Biophysics
A.B. Kenyon College 1958; Ph.D. Rockefeller University 1963
long interest in the physical aspects of membrane transport
processes—especially in phenomena related to charge transport—focuses now on
proton “pumps,” proton-coupled cation transporters, and potassium channels in
the plasma membranes of fungi, particularly Saccharomyces cerevisiae, Candida albicans (a pathogen), and Neurospora
crassa. Structure-function analysis of proteins
in these organisms is now possible, because of complete genome sequencing, and
the laboratory is using site-directed mutagenesis and heterologous expression
to analyze functional differences among analogous proteins in the three
species. Novel and surprising
properties have emerged, for example the development of enormous steady-state
membrane voltages (exceeding 350 mV), mediation of active potassium transport
by coupling to proton movements, and chloride channeling through potassium
transporters. Also, a major
current line of investigation is into the mechanisms by which small cationic
peptides (so-called RAMPs) produced by a wide variety of plant and animal
cells, become lethal to microorganisms, serving thereby as prototypes for new
classes of therapeutic drugs.
A., Slayman C.L., & Kuroda, T., 2005.
Quantitative modeling of chloride conductance in the TRK potassium
transporters of Saccharomyces cerevisiae. Biophys. J. 89:2412-2426.
Miranda, M., Bashi, E., Vylkova, S., Edgerton, M., Slayman, C., & Rivetta, A., 2009.
Conservation and dispersion of sequence and function in fungal TRK potassium transporters:
Focus on Candida albicans. FEMS Yeast Res. 9:278-292.
Baev, D., Rivetta, A., Vylkova, S., Sun, J.N., Zeng, G.-F., Slayman, C.L., & Edgerton, M., 2004.
The TRK1 potassium transporter is the critical effector for killing of Candida albicans by the
cationic protein, Histatin 5. J. Biol. Chem. 279: 55060-55072.
Smith, K.D., Gordon, P.B., Rivetta, A., Allen, K.E., Barbasova, T., Slayman, C., & Strobel, S.A., 2015.
Yeast FEX1 is a constitutively expressed fluoride channel with functional asymmetry of its two homologous domans.
J. Biol. Chem. 290:19874-19887.
Pardo, J.P., González-Andrade, M., Allen, K., Kuroda, T., Slayman, C.L., & Rivetta, A., 2015.
A structural model for facultative anion channels in an oligomeric membrane protein: the yeast TRK (K+) system.
Pflügers Arch.— Europ.J.Physiol. Oct. 2015.
Education & Training
|PhD||Rockefeller University (1963)|